Methods of isolation of bacteria microbiology module microbiology notes 10 methods of isolation of bacteria 10. Pdf isolation and characterization of bacteria from dye. We successfully isolated a novel aerobic chemolithotrophic sulfuroxidizing bacterium, designated strain so07, from wastewater biofilms growing under microaerophilic conditions. Oct, 2011 isolation and identification of lignin degrading bacteria. Isolation and characterization of lactic acid bacteria from dairy effluents barnali ashe and subhankar paul department of biotechnology and medical engineering, national institute of technology, rourkela, orissa, india received january 16, 2010 accepted may 24, 2010 abstract. Eight strains of bacteria were isolated from municipal sludge under aerobic or anaerobic conditions at 37c. Isolation and characterization of zinc resistant bacteria from a coil coating industrial wastewater treatment plant joshua b. A phosphate buffered mineral salts medium, ph 7, mm supplemented with 5 gl of nondialysed kraft lignin, 0.
Oilcontaminated wastewater samples were collected in ouagadougou city burkina faso and then used as source of bacterial isolation. In our study the sampling sites are the three nearby surroundings of madina tannery which is the largest tannery located at jalalabad area, near oxygen point in chittagong, bangladesh. Isolation and characterization of an nhexadecane degrading. Methods enterobacteriaceae and aeromonads were isolated at different stages of the wastewater treatment process and screened for the presence of integrase genes by dotblot hybridization. Isolation and characterization of arsenic reducing.
Isolation and characterization of heavy metalsresistant. High lipid fats and oils concentration contained in wastewater inhibits the activity of microbes in biological wastewater treatment systems. Isolation and characterization of lipiddegrading bacteria in. Characterization of bacterial communities in wastewater with. In this paper, we report the isolation, partial characterization, and in situ detection of a novel aerobic chemolithoautotrophic sulfuroxidizing bacterium inhabiting wastewater bio. Jun 23, 2017 ram c, ram nb, atya k, hemant jp 2009 isolation and characterization of potential aerobic bacteria capable for pyridine degradation in presence of picoline, phenol and formaldehyde as copollutants. Isolation and characterization of phaproducing bacteria from. Only three bacterial isolates mnz1, mnz4 and mnz6 were able to grow in high concentrations of arsenic. Appropriate amounts of samples were inoculated to a mineral salt medium ms with total quartz 9000 oil as sole carbon and energy source and then incubated for enrichment, prior to microbe isolation.
The isolates were cultured in a medium with different concentrations of copper cu, chromium cr, nickel ni, zinc zn and lead pb. The lipids degradation capability of lipiddegrading bacteria was investigated for possible application in treatment of lipidscontaminated wastewater. The isolate was identified as bacillus aryabhattai based on biochemical analysis and 16s rrna gene sequencing. Isolation of microorganism from dairy effluent for activated. Culturedependent techniques have been previously used for the isolation of a wide range of heterotrophic bacteria from activated sludge. Pdf isolation and characterization of bacteria from waste water. Isolation, characterization, and in situ detection of a. Various studies were done by many workers on the microbiological and biochemical characterization dairy wastewater of organized sector. Isolation and characterization of bacteria isolated from. Another simple method is by deepfreezing of the bacterial culture, stocked in a. In particular, wastewater from pharmaceutical plants could play a role in the selection of antibioticresistant bacteria in sewage. Some physiochemical parameters like ph, temperature, dissolved oxygen and arsenic imagem aqui gml were measured. Isolation and characterization of filamentous bacteria from a.
For the isolation of arsenic resistant bacteria, wastewater samples were collected from kala shah kakoo, pakistan in screw capped sterilized bottles. Isolation and characterization of bacteria from sewage and pond. Master of science in chemistry thesis, department of chemistry, rochester institute of technology, rochester, 108 p. Isolation and characterization of a lipiddegrading bacterium. The bacteria are allowed to grow before storing in the refrigerator or are covered with paraffin oil and stored at room temperature in the dark. Pdf isolation and characterization of arsenic resistant.
Isolation, characterization, and identification of. Isolation and characterization of filamentous bacteria. Hekeu department of biological sciences, microbiology unit, school of natural and applied sciences, college of science and technology, covenant university, p. Isolation and characterization of arsenic resistant bacteria. Isolation and characterization of aerobic denitrifying bacteria. Isolation and molecular characterization of biofouling. Isolation, screening and characterization of bacteria having. The isolate was identified by microbial identification system as pseudomonas stutzeri.
The isolation and characterization of heavy metal resistant bacteria is significant for its metal accumulation capability along with its resistance capacity. Thirty five soil samples were collected from waste effluent sites, serially diluted and spread. Singlefactor and response surface methodology experiments were used to optimize the culture medium and conditions. Kalarani 1 1department of biotechnology, sri padmavati mahila visvavidyalayam, womens university, tirupati 517 502, andhrapradesh, india.
Isolation, characterization and molecular identification of. Incidence and molecular characterization of multidrug. Pdf isolation and characterization of cdresistant bacteria. Isolation and characterization of arsenic resistant bacteria from wastewater article pdf available in brazilian journal of microbiology 454. Elkhatib c d a department of biotechnology and life sciences, faculty of post graduate studies for advanced sciences, benisuef university, benisuef, egypt. Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and nonhospital environments, northwest ethiopia, bmc research notes, 2014, pp. Rafatullah1 1division of environmental technology, school of industrial technology, universiti sains malaysia, penang, malaysia. Apr 04, 2019 isolation and characterization of mercuryresistant bacteria from wastewater sources in egypt martha m. On the basis of their physiological and biochemical characters, and in comparison with different. Isolation and characterization of bacteria from waste water sample and its pcr based identification through 16s rrna. Isolation and characterization of multidrug resistant. The aim of this study was to isolate and identify environmental bacteria from various raw water sources as well as the drinking water distributions system in mafikeng, south africa, and to determine their antibiotic resistance profiles. A polyhydroxyalkanoate phaproducing strain was isolated from propylene oxide po saponification wastewater activated sludge and was identified as brevundimonas vesicularis ujn1 through 16s rdna sequencing and biolog microbiological identification.
This study isolated and characterized thfdegrading bacteria to develop a biological process of 1,4dioxanecontaining wastewater treatment. Isolation and characterization of cdresistant bacteria. Here we demonstrated the isolation of a high efficiency atrazine. Request pdf isolation, characterization, and identification of bacteria from activated sludge and soluble microbial products in wastewater treatment systems activated sludge process is the. The present study proposed the isolation of arsenic resistant bacteria from wastewater. Isolation and characterization of aerobic denitrifying. In the present investigation, five novel crvi reducing bacteria were isolated from tannery effluents and solid wastes and identified as kosakonia cowanii mkpf2, klebsiella pneumonia mkpf5, acinetobacter gerneri mkpf7, klebsiella variicola mkpf8 and serratia marcescens mkpf12 by 16s rdna gene sequence analysis. Cod reduction ability of microorganisms isolated from highly loaded pharmaceutical wastewater pretreatment process.
Mar 21, 2018 a polyhydroxyalkanoate phaproducing strain was isolated from propylene oxide po saponification wastewater activated sludge and was identified as brevundimonas vesicularis ujn1 through 16s rdna sequencing and biolog microbiological identification. Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation. The implementation of wastewater treatment plants has been so far a challenge for most. This study reports the degradation and decolourization capability of a manganese peroxidase enzyme producing bacterium isolated from pulp and paper mill wastewater. The maximum tolerance concentration of crvi as k2cr2o7 of the bacterial. Characterization of bacterial communities in wastewater. The most common wastewater treatment systems used in mexico to treat this kind of effluents are activated sludge and aerated lagoons. Isolation and characterization of metalresistant bacterial.
Among five thfdegrading bacteria that were isolated, strain t1 from landfill soil and strains t3 and t5 from activated sludge showed stable cometabolic degradation of 100 mgl of 1,4dioxane when. Heavy metals resistant bacteria were obtained from wastewater samples in mining sites of itogon, benguet, philippines. The minimum inhibitory concentrations of arsenic against mnz1, mnz4 and mnz6 were 300 mgl, 300 mgl and 370 mgl respectively. Isolation and characterization of arsenic resistant. Prevalence and characterization of integrons from bacteria. Isolation, characterization and identification of microorganisms from. Isolation and characterization of filamentous bacteria from a fullscale municipal wastewater treatment plant in dubai.
The importance of bacterial isolates from waste water environment as a reservoir of antibiotic resistance and a potential source of novel resistance genes to clinical pathogens is underestimated. For isolation, the use of elemental sulfur s 0, which is the most abundant sulfur pool in the wastewater biofilms, as the electron donor was an effective measure to establish an enrichment culture of strain so07 and further isolation. For isolation, the use of elemental sulfur s, which is the most abundant sulfur pool in the wastewater biofilms, as the electron donor was an effective measure to establish an enrichment culture of strain so07 and. Materials and methods sampling site and isolation conditions. Isolation and characterization of bacteria from dye wastewater treating down flow fixed film reactor dffr isolation and characterization of bacteria from dye. Isolation and characterization of bacteria isolated from municipal. Bioaugmentation is an improvement in biological treatment processes, using isolated degrading strains zhu et al. The bacterium isolated could degradate nitrate nitrogen, nitrite nitrogen and amonium salt nitrogen. Isolation, identification and characterization of pharmaceutical. A suspension of 1% vv soil or wastewater samples from grease traps were incubated with the screening medium at 30c and 180 rpm for 72 h. This bacterium was able to reduce 67% and 54% colour and lignin.
The mar indices for the isolated sewage wastewater bacteria. Further biochemical characterization must be carried out if there is a need to. An aerobic denitrifying bacterium was isolated from sludge in wastewater pool. Isolation and characterization of bacteria from sewage and pond water, malda, india. Isolation and characterization of antibiotic resistance bacteria in hospital effluents article pdf available january 20 with 2,383 reads how we measure reads. For isolation, the use of elemental sulfur s, which is the most abundant sulfur pool in the wastewater biofilms, as the electron donor was an effective measure to establish an enrichment culture of strain. Isolation and characterization of cdresistant bacteria from industrial wastewater. The main idea behind this series is the subdivision of the original book into smaller books, which could be more easily purchased and used. Characterization of culturable bacteria from pulp and. Isolation and characterization of zinc resistant bacteria.
Isolation, characterization and molecular identification of bacteria from tannery effluent using 16s rrna sequencing p. This study is aimed at to isolate and characterize public health important bacteria from waste water in hospital and non hospital environments and evaluate the distribution of multiple drug. The identification is required so as to cure the illness or the infection caused due to. Isolation and characterization of tetrahydrofuran degrading. These authors reported an additional 10% increase in the removal of cod from the effluent, indicative of. Isolation and characterization of chromiumvireducing. Thesis submitted in fulfillment of the requirements for the degree of. As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant wwtp. Isolation and characterization of a lipiddegrading. Isolation and characterization of efficient cod degrading bacteria. One hundred and two bacterial isolates were isolated from 43 vegetable oil contaminated wastewater.
Isolation of most frequently occurring micro organisms from dairy wastewater and dairy sludge samples. Isolation and characterization of hydrocarbondegrading. Isolation and characterization of phaproducing bacteria. To investigate the presence and distribution of integroncarrying bacteria from a slaughterhouse wastewater treatment plant wwtp. In addition, characterization of individual microbial genera will also give important information about bacteria involved in biofouling and therefore aid the design of control strategies. Isolation and characterization of organic matterdegrading. Three strains identified as bacillus cereus, pseudomonas synxantha, and pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to. The isolated bacteria from the wastewater soils were found to be mainly of morganella morganii and bacillus cereus and these isolated bacteria were found to be very resistant to a wide range of antibiotics and heavy metals. Apr 05, 2014 in particular, wastewater from pharmaceutical plants could play a role in the selection of antibioticresistant bacteria in sewage. Characterization of bacterial communities in wastewater with enhanced taxonomic resolution by fulllength 16s rrna sequencing. Isolation and characterization of mercuryresistant bacteria. Isolation and biochemical characterization of heavymetal. Isolation and identification of bacteria from activated.
As inoculum material, soil collected from beneath decomposing wood. Pdf isolation and characterization of antibiotic resistance. Pdf isolation and characterization of phenol degrading. Isolation and characterization of multiple drug resistance. Isolation, selection, and biological characterization. Isolation of soil bacteria soil samples were collected from hospital wastewater sites of aizawl, mizoram. A microbial study was conducted from wastewater soils of hospitals in aizawl, mizoram, india which were in existence for a longtime. Characterization of culturable bacteria from pulp and paper industry wastewater, with the potential for degradation of cellulose, starch, and lipids ana m. Isolation and characterization of arsenic reducing bacteria from industrial effluents and their potential use in bioremediation of wastewater farah r. This study deals with the characterization of the bacterial strain isolated from wastewater and testing its resistance to different heavy metal concentrations and antibiotics. Isolation and characterization of arsenic resistant bacteria from wastewater syed zaghum abbas1,2, mehwish riaz2, naseem ramzan2, m. Department of natural science and public health, college of arts and sciences, zayed university, p.
The soil bacteria were enriched in minimal media for 24 hours at 37. In order to design an efficient biological waste water treatment it is important to know the microbiota composition of the wastewater and to identify the strains which. Mar 04, 2015 the present study proposed the isolation of arsenic resistant bacteria from wastewater. Water samples from five different sites raw and drinking water were analysed for the presence of faecal indicator bacteria as well as aeromonas and waste water environment as a reservoir of antibiotic resistance and a potential source of novel resistance genes to clinical pathogens is underestimated. Isolation, characterization, and in situ detection of a novel. Wastewater basics 101 target audience policy makers, leaders, and planners people who have a water quality agenda this presentation discusses the fundamentals of converting wastewater back to water how do we humans interact with the hydrologic cycle.
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